Technologies

Enzyme Technology

Kaneka has developed an extensive original enzyme library (wild type) as the result of many years of enzyme research and customer project collaboration. The most proven and useful enzymes from our enzyme library form the basis of what is called the “mutant enzyme library (Smart Library)”, which retains enzymes that have been modified to possess a range of reactions.
We are able to carry out screening for the optimal enzyme for the customer’s substrate from our original enzyme library and mutant enzyme library (Smart Library), and develop a scalable and robust enzyme process using the target enzyme.

Kaneka’s Enzyme Library expanded with Smart Library

Kaneka’s Enzyme Library expanded with Smart Library
  • Keto reductase
  • Ene reductase
  • Imine reductase
  • D-Amino acid dehydrogenase
  • L-Amino acid dehydrogenase
  • R-Transaminase
  • S-Transaminase
  • Amidase
  • Esterase
  • Imidase
  • Lipase
  • Hydantoinase
  • Decarbamoylase
  • Acylase
  • Succinylase
  • D-amino acid oxidase
  • Racemase
  • Cofactor recycling enzyme (GDH, FDH)
  • etc

What is Smart Library?

Effective Reshaping Active Center of Enzymes

'What is Smart Library?'→Choose template enzyme→High performance template enzyme→Structure analysis & homology modeling→Hot-spots search→Saturation mutagenesis on hot-spots→Smart Library(high quality mutant enzyme library)
Hot-spot search Active center

Kaneka’s Biotransformation Platform (Example)

Chiral Alcohol
Chiral Alcohol
Chiral Amine
Chiral Amine
Unnatural Amino Acid
Unnatural Amino Acid
3-Substituted 𝝪-Amino Acid
3-Substituted 𝝪-Amino Acid
Quaternary Amino Acid
Quaternary Amino Acid
C=C double bond reduction
C=C double bond reduction